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99
Thermo Fisher dna staining agent dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dna Staining Agent Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher to-pro-3
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
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Millipore dna probe dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dna Probe Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Vector Laboratories dna stain dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dna Stain Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Nikon 100× objective of a nikon e600 upright fluorescent microscope
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
100× Objective Of A Nikon E600 Upright Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher dapi solution
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dapi Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Partec)
90
Partec dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dapi, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Fisher Scientific dapi
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Dapi, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 4 6 diamidine 20 phenylindole dihydrochloride dapi roche
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
4 6 Diamidine 20 Phenylindole Dihydrochloride Dapi Roche, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ChemoMetec nucleocounter nc-3000 image cytometer
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
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99
Nikon eclipse ti2 fluorescent microscope
The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with <t>DAPI</t> and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed <t>DNA</t> is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.
Eclipse Ti2 Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with DAPI and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed DNA is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.

Journal: PLoS ONE

Article Title: The Effect of New Zealand Kanuka, Manuka and Clover Honeys on Bacterial Growth Dynamics and Cellular Morphology Varies According to the Species

doi: 10.1371/journal.pone.0055898

Figure Lengend Snippet: The effects of 4% (w/v) of a high-MGO honey (M3) and a high-hydrogen peroxide honey (MK1) on bacterial cellular morphology were examined. Overnight cultures of B. subtilis, E. coli, S. aureus and P. aeruginosa were treated with these honeys, cells collected at both lag and log phases of growth as indicated in , fixed with glutaraldehyde, stained with DAPI and imaged using fluorescence microscopy. All images are overlays of the phase-contrast image and the DAPI-stained (red) fluorescence image. The two left-hand panels show the no-honey treated control cells, the two middle panels M3 honey-treated cells, and the two right-hand panels show the MK1 honey-treated cells. In all images, condensed DNA is shown by green arrows; and dispersed DNA in B. subtilis cells is shown by blue arrows. An asterisk indicates lysed cells for B. subitlis (MK1, lag-phase cells). The scale bar represents 2 µm, except for S. aureus images, where it represents 1 µm.

Article Snippet: Harvested cells were treated for microscopy as described previously but with the following modifications: 20 μL of fixed cells were diluted 1∶1 with the DNA staining agent DAPI (4',6-diamidino-2-phenylindole; Life Technologies), to give a final DAPI concentration of 0.4 μg/mL for E. coli , B. subtilis and P. aeruginosa , and 0.8 μg/mL for S. aureus .

Techniques: Staining, Fluorescence, Microscopy, Control